Protein G Agarose
Protein G Agarose Sources Online
Protein G Agarose
Copyright 2005 - 2007
All Rights Reserved








Protein G Agarose Updates and Information

ICL Lab - antibodies epitope tag dog rat mouse crp anti flag ha myc 6xhis V5...
... Antibodies were immunoaffinity purified off protein G agarose. Western Blots 1:5,000 ˝ 1:20,000 ELISA Capture= 1:200-1:500 Application For Research and Manufacturing Only; Not For Use in Diagnostic ...
http://www.icllab.com/com ...t.jsp?product_id=1790


Promega - FAQs
... IgY does not bind to staphylococcal protein A or streptococcal protein G. The Immobilized Anti-Chicken IgY is an affinity purified agarose conjugate designed to immunoprecipitate IgY bound proteins (1 ...
http://www.promega.com/faq/eggfaq.html


Commissural neuron identity is specified by a homeodomain protein, Mbh1, tha...
... Immunocomplexes were pulled down using protein A and protein G-agarose beads for rabbit and rat IgGs, respectively. A 502 bp fragment spanning nucleotides +5507 to +6009 containing the E-box of the ...
http://dev.biologists.org ...ntent/full/132/9/2147


MMD - Matter and Material Devices Meeting
... saccharide and saccharide--protein samples A Jun 22th 24 ... in gas B Jun 23th 83 Giusfredi G. Absolute frequency measurements ... ice A Jun 22th 38 Gottardi R. Agarose gels: a valid model material ...
http://www.mmd.infm.it/poster_sessions.php


CytoExtr
... 5x). Load samples onto a 1.5% agarose gel, run at about 4V per cm ... assay Radioactive nuclei (e.g from ALVA31 cells) were prepared ... in cytoplasmic extracts (final protein concentration 7.5 mg/ml) in ...
http://www.celldeath.de/apometh/cfsystem.html


VIDO - Solutions Through Research
... determined. For some bacteria, however, fimbriae play a role in infection. See e.g. Normark et al. (1986) in Protein-carbohydrate Interactions in Biological Systems (D. Lark ed., 1986) pp. 3-12 ...
http://www.vido.org/paten ...atentnumber=5,849,531


Cell research (2004); 14(2):148-154
... Anti-MKK3, anti-ERK1, anti-p38, anti-HA, anti-H-Ras antibodies, anti-ÂČ-actin and Protein A/G plus-agarose were purchased from Santa Cruz Biotechnology. Inc.(Santa Cruz, CA). Anti-phospho-MKK3 ...
http://www.cell-research.com/20042/148.htm


JBC -- Cao et al. 276 (27): 24506
... After 1 h, 20 Ál of protein G-agarose slurry (Pierce) was added and incubation was continued for an additional 30 min. Protein G-agarose beads were recovered by centrifugation, washed three times in ...
http://www.pkclab.org/PKC ...2001-JBC-Jongstra.htm


A novel human G protein-coupled receptor is over-expressed in prostate cancer
... products were analyzed in 1.5% agarose gels stained with ethidium ... two GPCRs are very low, a protein motif search with TMHMM (a ... NCBI (build 30) databases (I.H.G.S.C., 2001; Venter et al., 2001 ...
http://www.funpecrp.com.b ...cob0005_full_text.htm


┴ţó┬ý┼┴ă╚ňĐ_A
... 1kb Marker+20X TAE(2L) NT2520 Agarose LE(50g)+1kb Marker+5X TBE(4L ... 20-2 Protein Experiment, For Protein Purification ── H 8 N 2 O 4 ... B binds to sterols (e. g. cholesterol, cholestanol) with ...
http://www.mdbio.com.tw/productlist_A.htm


IBD News: RedNova News - Health - Bovine Glycomacropeptide Is Anti-Inflammat...
... 14) and expressed as U/g protein. Total glutathione content ... 5'-CCA TTG GAG GGC AAG TCT GGT G-3'; antisense 5'-CGC CGG TCC AAG ... was resolved in 2.5% (wt:v) agarose gels using ethidium bromide ...
http://www.ibdnews.com/20 ...ws-health-bovine.html


Diagnostic Services - AVC
... HCO2 mmol/L 24+4 24+4 24+4 24+4 24+4 24+4 Base Excess 0+3 0+3 0+3 0+3 0+3 0+3 PROTEIN ELECTROPHORESIS (agarose gel) Albumin g/L 24-40 26-44 24-36 29-38 19-24 28-34 Alpha 1 g/L 2-4 13-18 N/A 7-13 9-12 2 ...
http://www.upei.ca/~diagserv/cp.htm


ListServ Archive: Biochemical Methods
... take 50 Ál of Protein G agarose bead-slurry per condition/point and wash 3-4x with RIPA. pellet at 13 krpm for 30 sec. pre-clear: take washed agarose beads + ~2 Ág of pre-clear Ab (whatever control ...
http://www.dictyostelium. ..._archive_biochem.html


Bio-Nobile -
... method for cutting gel pieces from any type of agarose gels. NEW PRODUCT: QuickPick˘ SML Plant DNA ... ... PickPenĂ magnetic tools. MagaCellĂ Protein A, Protein G or Streptavidin ˝coupled magnetizable ...
http://www.bio-nobile.com/about/newsarchive.asp


Laboratory of Receptor Biology: Bibliography
... of beta-adrenergic binding protein, in Frontiers in ... O'Hara, D. and Lefkowitz, R.J. Agarose-norepinephrine derivatives ... R.J., Levine, R. and Marinetti, G.V. Insulin activity: The solid ...
http://www.lefkolab.org/Bibliography.82.0.html


Retrovirology | Full text | HIV-1 Tat interacts with LIS1 protein
... Protein (G) and protein (A) agarose were purchased from Sigma (Atlanta, GA). Antibodies Anti-Tat ... ... Then protein G-agarose beads preblocked with 5% BSA and suspended in TNN buffer (50 mM Tris-HCl (pH ...
http://www.retrovirology.com/content/2/1/6


ProFound˘ HA and C-Myc Tagged Protein Kits Enable IP or co-IP of Proteins
... covalent linkage between the antibody and the agarose results in a final IP/co-IP product that is ... ... background that often occurs when Protein A or Protein G is used. The kits include all necessary ...
http://www.piercenet.com/ ...9D2-B427-261427760FD4


Aris Lab Methods
... and NEPHGE Agarose Gel Agarose RNA Formaldehyde Agarose RNA Glyoxal Large Urea PAGE Mini PAGE PAGE Gel ... ... DEAE Antibody Preabsorbtion Antibody X-Link Protein G EM: EM Immuno Probe EM Yeast Cells Yeast EM ...
http://plaza.ufl.edu/johnaris/methods.html


Saleh, Mol Vis 2001; 7:240-246.
... To reduce the non-specific adsorption, the supernatant was pre-cleared by incubation with protein G agarose beads and pre-immune sera (1:1000) in 2.5% bovine serum albumin (BSA) at 4 C for 15 min ...
http://www.molvis.org/molvis/v7/a34/


Bibliography
... elution from poly-L-proline agarose columns. Cell Motil. Cytoskel ... T.D. (1990) The actin-binding protein profilin binds to PIP2 and ... J.W., Machesky, L.M., Rhee, S.G. and Pollard, T.D. (1991 ...
http://www.yale.edu/pollard_lab/bibliography1.htm


Research and Findings - Main Research Findings About Zeaxanthin & Lutein
... 0.15ug per g protein). Birds fed with the higher dietary Z supplement for 6 or 12 months had significantly higher lens Z than birds fed lower or no dietary Z (P<0.0001). Lens L was not altered by ...
http://www.chrysantis.com/findings.htm


Biochemicals S Proteins Spermidine thru Synapsin
... Streptavidin Agarose IP01CB Streptavidin Agarose Solution 69219CB Streptavidin AP Conjugate ... ... Phycoerythrin Conjugate 571125CB Streptavidin, Protein G Conjugate 189738CB Streptavidin, TEXAS RED ...
http://www.vgdusa.com/biochemicals_S.htm


[Full text] High level expression of apoptosis inhibitor in hepatoma cell li...
... IAPs RNA Ten micro grams RNA was resolved by 1% agarose gel electrophoresis. RNA was transferred to ... ... cell lysate was immune-precipitated with 30║l protein G beads (Roche, Switzerland) at 4 C overnight ...
http://www.medsci.org/v02p0030.htm


Alzforum: Live Discussions: Generation of an apoptotic intracellular peptide...
... X100. After a preclearing step with 40ml/ml of protein G-agarose, the extracted proteins were immunoprecipitated with the monoclonal antibody 643-695 /Jonas (10mg/ml) for 3 hours at 4C. Then 10ml of ...
http://www.alzforum.org/res/for/journal/Dadamio/


Nm23-H1 Metastasis Suppressor Expression Level Influences the Binding Proper...
... and 2 (Sigma), and 1 mM phenylmethylsulfonyl fluoride, and the lysates were precleared with protein G-agarose (Roche Molecular Biochemicals) for 1 h at 4C. Eight hundred micrograms of lysate was ...
http://mcb.asm.org/cgi/content/full/25/4/1379


A transmembrane CXC chemokine is a ligand for HIV-coreceptor Bonzo
... of the clones showed a mouse protein with 71% amino acid sequence ... 10 g/ml of leupeptin and 10 g/ml of apoprotin. Flag-tagged ... with anti-Flag agarose (Sigma), resolved by 12.5 ...
http://www.cgen.com/resea ...tions/nature1000.html


Newsletter Lucerna-Chem
... IPeX technology eliminates Protein A or Protein G agarose beads. Immune complex is formed when crude samples is incubated with the beads covalently bound antibody. Elution step dissociated the bound ...
http://www.lucerna-chem.c ...8/newsletter2_08.html


Protein Purification - Biocompare Buyer's Guide
... Media Protein A Protein G Chloroplast Isolation Kits ... Fractionation Kits Soluble Protein Fractionation Kits Freeze ... Flasks Fusion Tag Removal Agarose Bead Removal Cleavage Control ...
http://www.biocompare.com ...ein-Purification.html


Group Publications
... C4 epimerase fusion proteinţ Chem. Commun. 2003 , (12 ... Zhang, J.-B.; Chen, X.; Wang, P. G. "Combined Biosynthetic Pathway ... Enzymes Immobilized on Agarose Beads" ChemBioChem 2002, 3 ...
http://wang.biosci.ohio-s ...edu/publication3.html


PROTIGA - Services
... Metal binding: His-tagged proteins - Protein G/A binding: Antibodies - Ligand binding ... ... studies 3. Immobilization of proteins to agarose beads: - Applications: Antibody purification ...
http://www.protiga.com/services.htm


Resources About Protein G Agarose

Click Here for Articles

Copyright 2005 - 2007
All Rights Reserved
Partner Sites